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rabbit anti arl13b  (Proteintech)


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    Structured Review

    Proteintech rabbit anti arl13b
    Rabbit Anti Arl13b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+arl13b/ARL13B+Antibody/pm41872878-471-16-18
    Average 96 stars, based on 952 article reviews
    rabbit anti arl13b - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Epithelial Dynamics of Cystogenesis in Genetic Models of Autosomal Dominant Polycystic Kidney Disease.
    Article Snippet: .. Sections were incubated with the following primary antibodies: rabbit anti-Arl13b (1:200, 17711-1-AP, Proteintech, Donghu, Wuhan, China) and mouse anti-Aqp2 (1:50, sc-515770, Santa Cruz, Dallas, TX, USA). .. After washing, sections were incubated with appropriate secondary antibodies: goat anti-rabbit IgG (H + L)-Alexa Fluor 594 (cross-adsorbed, Invitrogen, A11008, Waltham, MA, USA) and goat anti-mouse IgG (H + L)-Alexa Fluor 488 (cross-adsorbed, Invitrogen, A32728, Waltham, MA, USA).

    Article Title: The rs713586 risk variant dysregulates ADCY3 rather than DNAJC27, leading to obesity through ZFP42-TET1-mediated DNA methylation
    Article Snippet: .. Following three washes with PBS, cells were permeabilized with 0.2% Triton X-100 in PBS for 15 min. After blocking in 10% goat serum in PBS, cells were incubated overnight at 4 °C with primary antibodies: rabbit anti-ARL13B (RRID: AB_2060867 ; Proteintech, 17711-1-AP, 1:1000) and mouse anti-acetylated tubulin (RRID: AB_477585 , Sigma, T6793, 1:2000). .. The next day, after three PBS washes, cells were incubated with Alexa Fluor488- and 594-conjugated secondary antibodies (1:1000; Thermo Scientific) for 2 h, and then mounted with an anti-quenching sealing agent (0100-01, Southern Biotech).

    Article Title: The rs713586 risk variant dysregulates ADCY3 rather than DNAJC27, leading to obesity through ZFP42-TET1-mediated DNA methylation.
    Article Snippet: .. Following three washes with PBS, cells were permeabilized with 0.2% Triton X100 in PBS for 15 min. After blocking in 10% goat serum in PBS, cells were incubated overnight at 4 ◦ C with primary antibodies: rabbit anti-ARL13B (RRID: AB_2060867; Proteintech, 17711-1-AP, 1:1000) and mouse anti-acetylated tubulin (RRID: AB_477585, Sigma, T6793, 1:2000). .. The next day, after three PBS washes, cells were incubated with Alexa Fluor488- and 594-conjugated secondary antibodies (1:1000; Thermo Scientific) for 2 h, and then mounted with an antiquenching sealing agent (0100-01, Southern Biotech).

    Article Title: Epithelial Dynamics of Cystogenesis in Genetic Models of Autosomal Dominant Polycystic Kidney Disease
    Article Snippet: .. Sections were incubated with the following primary antibodies: rabbit anti-Arl13b (1:200, 17711-1-AP, Proteintech, Donghu, Wuhan, China) and mouse anti-Aqp2 (1:50, sc-515770, Santa Cruz, Dallas, TX, USA). .. After washing, sections were incubated with appropriate secondary antibodies: goat anti-rabbit IgG (H + L)-Alexa Fluor 594 (cross-adsorbed, Invitrogen, A11008, Waltham, MA, USA) and goat anti-mouse IgG (H + L)-Alexa Fluor 488 (cross-adsorbed, Invitrogen, A32728, Waltham, MA, USA).

    Immunofluorescence:

    Article Title: Primary cilium and TULP3-dependent ciliary targeting of ACE2 in SARS-CoV-2 tropism.
    Article Snippet: Fluorescence images were captured using either Nikon C2 Plus confocal microscope or Nikon AX R confocal microscope. .. The primary antibodies used for the immunofluorescence staining include: rabbit anti-ARL13B, 1:1000 (17,711–1-AP, Proteintech); mouse anti-ARL13B, 1:1000 (#N295B/66, Developmental Studies Hybridoma Bank); mouse anti-Acetylated-∝-tubulin, 1:4000 (T6793, Sigma-Aldrich); mouse anti-polyglutamylated-tubulin clone GT335, 1:500 (AG-20B-0020-C100, AdipoGen); goat anti-ACE2, 1:1000 (AF933, R&D Systems); rabbit anti-TULP3, 1:200 (13,637–1-AP, Proteintech), rabbit anti-SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, 1:500 (40,150-R007, Sino Biological). .. Rabbit anti-NRP1 antibody, 1:500 (ab81321, Abcam).

    Staining:

    Article Title: Primary cilium and TULP3-dependent ciliary targeting of ACE2 in SARS-CoV-2 tropism.
    Article Snippet: Fluorescence images were captured using either Nikon C2 Plus confocal microscope or Nikon AX R confocal microscope. .. The primary antibodies used for the immunofluorescence staining include: rabbit anti-ARL13B, 1:1000 (17,711–1-AP, Proteintech); mouse anti-ARL13B, 1:1000 (#N295B/66, Developmental Studies Hybridoma Bank); mouse anti-Acetylated-∝-tubulin, 1:4000 (T6793, Sigma-Aldrich); mouse anti-polyglutamylated-tubulin clone GT335, 1:500 (AG-20B-0020-C100, AdipoGen); goat anti-ACE2, 1:1000 (AF933, R&D Systems); rabbit anti-TULP3, 1:200 (13,637–1-AP, Proteintech), rabbit anti-SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, 1:500 (40,150-R007, Sino Biological). .. Rabbit anti-NRP1 antibody, 1:500 (ab81321, Abcam).

    Blocking Assay:

    Article Title: The rs713586 risk variant dysregulates ADCY3 rather than DNAJC27, leading to obesity through ZFP42-TET1-mediated DNA methylation
    Article Snippet: .. Following three washes with PBS, cells were permeabilized with 0.2% Triton X-100 in PBS for 15 min. After blocking in 10% goat serum in PBS, cells were incubated overnight at 4 °C with primary antibodies: rabbit anti-ARL13B (RRID: AB_2060867 ; Proteintech, 17711-1-AP, 1:1000) and mouse anti-acetylated tubulin (RRID: AB_477585 , Sigma, T6793, 1:2000). .. The next day, after three PBS washes, cells were incubated with Alexa Fluor488- and 594-conjugated secondary antibodies (1:1000; Thermo Scientific) for 2 h, and then mounted with an anti-quenching sealing agent (0100-01, Southern Biotech).

    Article Title: The rs713586 risk variant dysregulates ADCY3 rather than DNAJC27, leading to obesity through ZFP42-TET1-mediated DNA methylation.
    Article Snippet: .. Following three washes with PBS, cells were permeabilized with 0.2% Triton X100 in PBS for 15 min. After blocking in 10% goat serum in PBS, cells were incubated overnight at 4 ◦ C with primary antibodies: rabbit anti-ARL13B (RRID: AB_2060867; Proteintech, 17711-1-AP, 1:1000) and mouse anti-acetylated tubulin (RRID: AB_477585, Sigma, T6793, 1:2000). .. The next day, after three PBS washes, cells were incubated with Alexa Fluor488- and 594-conjugated secondary antibodies (1:1000; Thermo Scientific) for 2 h, and then mounted with an antiquenching sealing agent (0100-01, Southern Biotech).

    other:

    Article Title: The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down.
    Article Snippet: The following primary antibodies were used: rabbit anti-Arl13b (Proteintech, 17711-1-AP; IB: 1:2,000, IF: 1:1000), mouse antiacetylated α-tubulin (Ac-Tub) (Sigma-Aldrich, T6793; IF: 1:2000), mouse anti-γ-tubulin (γ-Tub) (Sigma-Aldrich, T6557; IF: 1:1000), mouse Tubulin β3 (TUJ1) (BioLegend [Covance], MMS-435P; IF: 1:500), chicken anti-GFP (Abcam, ab13970; IF: 1:1000), chicken anti-mCherry (Abcam, ab205402; IF: 1:1000), rabbit anti-RFP (Medical & Biological Laboratories, PM005; IB: 1:2,500, IF: 1:1000), and mouse anti-β-actin (Sigma-Aldrich, A5441; IB: 1:5000).

    Article Title: Whole-Mount Visualization of Primary Cilia in the Developing Mouse Brain
    Article Snippet: Rabbit anti-Arl13b (1:200) (Proteintech, catalog number: 17711-1-AP) 8.



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